Source protein: Protein A affinity chromatography purified protein from mouse ascites fluid. Clonality and Host Species : GST.B6, Mouse Immunogen and Isotype: GST protein, IgG2a Specificity and reactivity: Detects native and denatured forms of purified GST or GST fusion proteins. Application: Western blot, Dot blot, ELISA, IHC/IS, Western blot (with ECL) 1-5 ng of GST-tagged fusion protein. Available form: Lyophilized 1mg/mL (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN 3 ) Working procedure: Western blot (with ECL) 1:1000-3,000 dilution incubates for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest. Storage: Supplied lyophilized antibodies can be stored at 20 0 C for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20 o C for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4 0 C for several days to 2 weeks.
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