This hot-start PCR polymerase mix and special buffer are formulated to amplify DNA templates with high GC content or a significant amount of secondary structure. This combination allows synthesis of products up to 20 kb for complex templates, such as GC-rich genomic DNA, and up to 48 kb for less complex templates, such as lambda DNA. Sufficient polymerase mix and buffer are provided for 200 reactions of 25 µl each.
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